Assay of Montelukast Sodium (UV-VIS)Spectrophotometric Method

Assay of Montelukast Sodium (UV-VIS)Spectrophotometric Method.

Preparation of standard solution : Take 50mg of Montelukast Sodium in a 100ml volumetric flask, add 50ml of water and sonicate . Add 10ml of 1M Sodium

Hydroxide solution and dilute to 100ml with water. Transfer 1ml of this solution in a 50ml volumetric flask and dilute to 50ml with o.1N NaOH solution.

Preparation of sample solution :  Crush and finely powder 20 tablets. Weigh an amount of tablet powder containing 50mg of Montelukast Sodium in a 100ml volumetric flask, add 50ml of water and sonicate for about 10 minutes. Add 10ml of 1M NaOH soln. and dilute to 100ml with water, filter. Transfer 1ml of this solution in a 50ml volumetric flask and dilute to 50ml with 0.1N NaOH soln.

Measure the absorbance of the resulting solution at he maximum wavelength 345nm, using 0.1N NaOH as blank.

                                            Abs. of sample X Wt. of standard

% 0f Montelukast Sodium=---------------------------------------X potency of Standard

                                             Abs.of Std. X    Wt. of sample