Preparation of Standard Solution : Take 10mg.
of Atorvastatin Calcium Trihydrade in a 100ml volumetric flask, add 50ml of
Methanol and sonicate , dilute to volume with the same solvent. Transfer 5ml of
this solution in a 50ml Volumetric flask and dilute to volume with methanol.
Preparation of Sample Solution : Crush 20 tablets finely and weigh an amount of powder
tablet containing 10mg of Atotvastatin Calcium Trihydrade in a 100ml Volumetric
Flask ,add 50ml Methanol and sonicate , dilute to Volume with methanol and filter.
Transfer 5ml of the filtrate in a 50ml volumetric flask and make volume with
methanol.
Measure the absorbance of the
resulting solutionat the maximum wavelength at 247nm,using a methanol as blank.
Calculation :
Absorbance of sample x
weight of standard
% of Atorvastatin = ……………………………………………
X Potency of Standard
Absorbance of Std.x Weght of sample x 1.084
