Calibration of UV- VIS Detector for HPLC

1.0      PURPOSE:  To ensure smooth functioning of HPLC Machine. 2.0      SCOPE  :  The SOP applies to the calibration of UV-VIS detector of Shimadzu                          Company model No. SPD-10A vp. 3.0   DEFINITION:    Nil. 4.0 RESPONSBILITY:  Quality Control officer. 5.0  PROCEDURES: 5.1      Press & hold down the “shift” key & turn the power switch on. 5.2      Press the “func” key until lamp is displayed. 5.3      Press  “1”, then enter. 5.4      Press “func” until “Wave check” is displayed. 5.5      Press “enter”. The wavelength accuracy check is run automatically. CHECK GOOD should appear on the screen when the test ends.

Calibration of Spectrophotometer

1.0   PURPOSE:   To ensure smooth functioning of Spectrophotometer. 2.0   SCOPE   :   The SOP applies to the calibration of Spectrophotometer of Shimadzu                             Company model No. UV-1201 3.0   DEFINITION:  Nil. 4.0  RESPONSIBILITY :  QC officer. 5.0  PROCEDURE: 5.1  Switch on the machine & wait for auto calibration . 5.2  After auto calibration check the absorbance of potassium Dichromate Solution VS at the wavelengths indicated in the following table which gives for each Wavelength the exact value of A (1% 1cm) & the permitted limits. The tolerance of absorbance is ± 0.01           Wavelength (nm)                     A(1% 1cm)                     Maximum Tolerance                     235                        -                124.5           -                  122.9 – 126.2                  257                        -                 144.0           -                  142.4 – 145.2                  315                        -                 48.6            -                    47.0 – 50.3                  350                        -                 106.6           -                   104.9 – 108.2

Calibration of pH Meter

1.0      Purpose:  To ensure smooth running of PH meter with desired accuracy. 2.0   Scope    :   This SOP applies to the calibration of PH meter. 3.0   Definitions:  Nil 4.0      Responsibility:  QC officer. 5.0   Procedures    : 5.1          Place buffer 4.0 or 7.0 in the vessel & press CAL / MEANS after switching on the Machine. 5.2          Press CON button & enter the value selected for Calibration & record the reading. 5.3          The electrode of PH meter is immersed in the buffer solution of PH 4.0 & adjusting The meter to the appropriate PH value. Ref. BP-93  APPENDIX VM (Table-2) How to use the PH meter: 1)      Press the button CAL / MEANS 2)      Select the next higher PH buffer setting in the PH calibration mode. 3)      Select the next lower PH buffer setting in the PH calibration mode. 4)      Press CON button & enter the value selected for calibration.