Calibration of UV- VIS Detector for HPLC

1.0      PURPOSE:  To ensure smooth functioning of HPLC Machine. 2.0      SCOPE  :  The SOP applies to the calibration of UV-VIS detector of Shimadzu                          Company model No. SPD-10A vp. 3.0   DEFINITION:    Nil. 4.0 RESPONSBILITY:  Quality Control officer. 5.0  PROCEDURES: 5.1      Press & hold down the “shift” key & turn the power switch on. 5.2      Press the “func” key until lamp is displayed. 5.3      Press  “1”, then enter. 5.4      Press “func” until “Wave check” is displayed. 5.5      Press “enter”. The wavelength accuracy check is run automatically. CHECK GOOD should appear on the screen when the test ends.