Assay of Montelukast Sodium (UV-VIS)Spectrophotometric Method.
Preparation of standard
solution : Take 50mg
of Montelukast Sodium in a 100ml volumetric flask, add 50ml of water and
sonicate . Add 10ml of 1M Sodium
Hydroxide solution and dilute to 100ml with water. Transfer
1ml of this solution in a 50ml volumetric flask and dilute to 50ml with o.1N
NaOH solution.
Preparation of sample
solution : Crush and finely powder 20 tablets.
Weigh an amount of tablet powder containing 50mg of Montelukast Sodium in a
100ml volumetric flask, add 50ml of water and sonicate for about 10 minutes.
Add 10ml of 1M NaOH soln. and dilute to 100ml with water, filter. Transfer 1ml
of this solution in a 50ml volumetric flask and dilute to 50ml with 0.1N NaOH
soln.
Measure the absorbance of the resulting solution at he
maximum wavelength 345nm, using 0.1N NaOH as blank.
Abs. of sample X Wt. of standard
% 0f Montelukast
Sodium=---------------------------------------X potency of Standard
Abs.of Std. X Wt. of sample
