Calibration of Spectrophotometer

1.0   PURPOSE:   To ensure smooth functioning of Spectrophotometer. 2.0   SCOPE   :   The SOP applies to the calibration of Spectrophotometer of Shimadzu                             Company model No. UV-1201 3.0   DEFINITION:  Nil. 4.0  RESPONSIBILITY :  QC officer. 5.0  PROCEDURE: 5.1  Switch on the machine & wait for auto calibration . 5.2  After auto calibration check the absorbance of potassium Dichromate Solution VS at the wavelengths indicated in the following table which gives for each Wavelength the exact value of A (1% 1cm) & the permitted limits. The tolerance of absorbance is ± 0.01           Wavelength (nm)                     A(1% 1cm)                     Maximum Tolerance                     235                        -                124.5           -                  122.9 – 126.2                  257                        -                 144.0           -                  142.4 – 145.2                  315                        -                 48.6            -                    47.0 – 50.3                  350                        -                 106.6           -                   104.9 – 108.2